Flashcards · Biology
Cell Structure
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What is the main difference between eukaryotic and prokaryotic cells?
Eukaryotic cells have a nucleus enclosing their DNA. Prokaryotic cells have no nucleus.
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Which kinds of living things have eukaryotic cells?
Animals, plants, fungi and single-celled organisms such as Amoeba.
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What type of cell is a bacterium?
A prokaryotic cell.
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Where is the DNA in a bacterial cell?
In a single loop lying free in the cytoplasm.
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What is a plasmid?
A small extra ring of DNA in a bacterial cell, often carrying genes such as antibiotic resistance.
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What is the bacterial cell wall for?
It supports the cell and protects it. It is not made of cellulose.
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Name three structures found in both bacterial and animal cells.
Cell membrane, cytoplasm and ribosomes.
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Roughly how much bigger is an animal cell than a bacterium?
About 10 times: around 20 µm compared with around 2 µm.
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What does the prefix micro mean?
One millionth, so \(1\ \mu\text{m} = 1 \times 10^{-6}\text{ m}\).
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What does the prefix nano mean?
One billionth, so \(1\text{ nm} = 1 \times 10^{-9}\text{ m}\).
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Write 0.000 03 m in standard form.
\(3 \times 10^{-5}\text{ m}\).
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One cell is 100 times bigger than another. How many orders of magnitude is that?
Two, because \(100 = 10^{2}\).
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How many micrometres are there in 1 mm?
1000.
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What is the usual mistake about bacteria and DNA?
Thinking bacteria have no DNA. They do, but it is not enclosed in a nucleus.
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What is the structure marked X in this bacterial cell? A plasmid: a small extra ring of DNA that can carry genes such as antibiotic resistance.
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Label every structure of this bacterial cell.
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What does the nucleus do?
It contains the genetic material (DNA) and controls the activities of the cell.
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What happens in the cytoplasm?
Most of the cell's chemical reactions.
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What does the cell membrane do?
It controls which substances pass into and out of the cell.
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What is the function of mitochondria?
They are where aerobic respiration takes place, releasing energy for the cell.
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What do ribosomes do?
They make proteins.
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What is the cell wall of a plant cell made from?
Cellulose.
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What does the cell wall do?
It strengthens the cell and supports the plant.
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What do chloroplasts contain, and why?
The green pigment chlorophyll, which absorbs light for photosynthesis.
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What is the permanent vacuole filled with?
Cell sap, a solution of sugars and salts.
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Name three structures found in plant cells but not in animal cells.
Cell wall, chloroplasts and a permanent vacuole.
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Why do muscle cells contain many mitochondria?
Contraction needs a lot of energy, which mitochondria release in respiration.
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Why do root cells have no chloroplasts?
Roots are underground where there is no light, so photosynthesis cannot happen.
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Do mitochondria make energy?
No. They release energy from glucose in respiration. Energy cannot be made.
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What is the structure marked X, and what does it do? A mitochondrion. It is where aerobic respiration takes place, releasing energy for the cell.
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Name the structure marked X, and say what it does. A chloroplast. It contains chlorophyll, which absorbs light for photosynthesis.
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Name the large structure marked X in this plant cell. What does it contain? The permanent vacuole. It is filled with cell sap, a solution of sugars and salts.
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Label every structure of this plant cell.
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Write the magnification formula.
Magnification = size of image ÷ size of real object.
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How do you find the real size from the image size and the magnification?
Real size = image size ÷ magnification.
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What is resolution?
How clearly two points that are close together can be told apart.
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Which has higher magnification and resolution: light or electron microscope?
The electron microscope.
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Name two disadvantages of an electron microscope.
It is large and expensive, and the specimen must be dead.
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Name an advantage of the light microscope.
It is cheap and easy to use, and living cells can be viewed.
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A ×10 eyepiece and a ×40 objective lens are used. What is the total magnification?
×400.
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How many micrometres are in 1 mm?
1000.
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How many nanometres are in 1 µm?
1000.
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Why is a stain used on a slide?
It makes the cell structures easier to see.
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Which objective lens should you start with?
The lowest power one, which gives the widest view.
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Which wheel do you use to find the image, and which to make it sharp?
The coarse focus wheel finds it, then the fine focus wheel sharpens it.
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Why is a coverslip lowered at an angle?
To avoid trapping air bubbles.
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How should a biological drawing be done?
Sharp pencil, clear single lines, no shading, labelled, with the magnification written.
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Label every part of this light microscope.
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Name part X of the microscope and say what it does. The stage. It is the platform that holds the slide, kept in place by the clips.
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Name part X of the microscope and say what it does. The fine focus wheel. It makes small adjustments to give a sharp, clear image, after the coarse wheel has found it.
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What is a specialised cell?
A cell with a structure that is suited to its particular job.
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What is differentiation?
The process by which a cell changes to become specialised.
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Why does a sperm cell have a long tail?
So it can swim to the egg.
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Why does the middle of a sperm cell contain many mitochondria?
They release the energy needed for swimming.
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What is the job of the acrosome?
It contains enzymes that digest the outer layer of the egg so the sperm can get in.
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How is a nerve cell adapted to carry impulses over long distances?
It has a long axon, and a myelin sheath that insulates it.
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Why do nerve cells have branched dendrites?
They connect to many other nerve cells.
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What do muscle cells contain that lets them contract?
Special protein fibres that slide over each other, and many mitochondria for energy.
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How does the shape of a root hair cell help it?
The long projection gives a large surface area to absorb water and minerals.
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Why do root hair cells have a large permanent vacuole?
It speeds up the movement of water into the cell.
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What does xylem transport?
Water and mineral ions, from the roots up to the leaves.
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Why is xylem strengthened with lignin?
The rings stop the hollow tube collapsing and make it strong.
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What does phloem transport?
Dissolved sugars to wherever they are needed.
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What are sieve plates?
The walls between phloem cells, with holes that let sugars flow through.
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What is the job of companion cells in phloem?
Their mitochondria supply the energy for moving sugars.
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Do animal cells or plant cells keep the ability to differentiate throughout life?
Plant cells. Most animal cells differentiate early.
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Put these in order, smallest first: organ, cell, tissue, organism.
Cell, tissue, organ, organism.
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Name the part marked X on this sperm cell, and explain why it is there. Mitochondria in the middle section. They release the energy from respiration that the tail needs for swimming.
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Name the part marked X on this nerve cell and say what it does. The myelin sheath. It is a fatty layer that insulates the axon and speeds up the impulse.
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What is the structure marked X on this root hair cell, and how does it help? The root hair. Its long, thin shape gives a large surface area for absorbing water and mineral ions from the soil.
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How do bacteria multiply?
By binary fission: a cell divides into two identical cells.
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How quickly can bacteria divide?
As often as once every 20 minutes, with enough nutrients and a suitable temperature.
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What are the two ways of growing bacteria?
In nutrient broth, or on agar gel in a Petri dish.
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What is aseptic technique?
Methods used to stop unwanted microorganisms getting into a culture.
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Why are the Petri dish and agar sterilised?
To kill any microorganisms already on them.
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Why is the inoculating loop passed through a flame?
To kill microorganisms on it, so only the wanted bacteria are transferred.
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Why is the lid taped on?
To stop microorganisms from the air getting in.
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Why is the tape not run all the way round the dish?
So that oxygen can get in, which stops harmful anaerobic bacteria growing.
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What is the maximum temperature for incubating in school?
25 °C.
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Why is 25 °C the limit in school?
Higher temperatures make it more likely that harmful pathogens grow.
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A bacterium divides every 20 minutes. How many are there after 1 hour, starting from one?
8 (three divisions: 2, 4, 8).
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How do you find the number of divisions in a time?
Divide the time by the mean division time.
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What does a clear zone around a disc show?
The antibiotic or antiseptic has killed the bacteria or stopped them growing there.
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What does a bigger clear zone tell you?
The chemical is more effective.
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What is the formula for the area of a circle?
\(A = \pi r^{2}\), where r is the radius (half the diameter).
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Why is a control disc soaked in sterile water included?
To show that the paper itself does not stop the bacteria growing.
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Which antibiotic is most effective here, and how can you tell? Disc A. It has the biggest clear zone, so it killed or stopped the growth of the most bacteria.