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Exam questions · Biology

Respiration and Biological Molecules

  • 26 exam questions
  • 73 marks
  • 28 quick checks

Aerobic and Anaerobic Respiration

Just this lesson
  1. 1 State [1 mark]

    Respiration is described as an exothermic reaction. State what exothermic means.

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    Model answer

    It transfers energy to the surroundings.

    Mark scheme

    • Transfers energy to the surroundings — 1 mark
  2. 2 Name [3 marks]

    Figure 1 shows three types of respiration. Name the substances shown at A, B and C.

    Three respiration equations drawn with icons, with three substances marked A, B and C.
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    Model answer

    A is oxygen. B is lactic acid. C is ethanol.

    Mark scheme

    • A: oxygen — 1 mark
    • B: lactic acid — 1 mark
    • C: ethanol — 1 mark
  3. 3 Write [2 marks]

    Write the word equation for aerobic respiration.

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    Model answer

    Glucose + oxygen → carbon dioxide + water.

    Mark scheme

    • Glucose + oxygen as reactants — 1 mark
    • Carbon dioxide + water as products — 1 mark
  4. 4 State [3 marks]

    Give three uses of the energy that organisms transfer by respiration.

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    Model answer

    To build larger molecules from smaller ones, for movement and to keep warm.

    Mark scheme

    • Building larger molecules — 1 mark
    • Movement — 1 mark
    • Keeping warm — 1 mark
  5. 5 Compare [4 marks]

    Compare aerobic respiration and anaerobic respiration in muscles.

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    Model answer

    Aerobic respiration uses oxygen, but anaerobic respiration does not. Aerobic respiration makes carbon dioxide and water, but anaerobic respiration in muscles makes lactic acid. Aerobic respiration transfers much more energy than anaerobic respiration.

    Mark scheme

    • Aerobic needs oxygen, anaerobic does not — 1 mark
    • Aerobic makes carbon dioxide and water — 1 mark
    • Anaerobic makes lactic acid in muscles — 1 mark
    • Aerobic transfers much more energy — 1 mark
  6. 6 Explain [2 marks]

    Explain why anaerobic respiration transfers much less energy than aerobic respiration.

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    Model answer

    In anaerobic respiration the glucose is only partly oxidised. The oxidation of glucose is incomplete, so less energy is transferred.

    Mark scheme

    • Glucose is only partly oxidised — 1 mark
    • So much less energy is transferred — 1 mark
  7. 7 Explain [3 marks]

    Yeast is added to bread dough. Explain why the dough rises.

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    Model answer

    The yeast respires anaerobically, using sugar in the dough. This releases carbon dioxide. The carbon dioxide makes bubbles in the dough, which makes it rise.

    Mark scheme

    • Yeast respires anaerobically — 1 mark
    • Carbon dioxide is released — 1 mark
    • Bubbles of gas make the dough rise — 1 mark
  8. 8 Compare [3 marks]

    Compare anaerobic respiration in animal cells with anaerobic respiration in yeast cells.

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    Model answer

    Both start with glucose and both make only a small yield of ATP, 2 ATP per glucose. Animal cells make lactic acid. Yeast cells make ethanol and carbon dioxide.

    Mark scheme

    • Both use glucose and make 2 ATP per glucose — 1 mark
    • Animal cells make lactic acid — 1 mark
    • Yeast cells make ethanol and carbon dioxide — 1 mark
  9. 9 Explain [2 marks]

    Respiration is described as a universal chemical process. Explain what this means and what it supplies.

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    Model answer

    It happens continuously in all living cells, including plants, animals and fungi. It supplies ATP, which carries energy to where it is needed.

    Mark scheme

    • It happens in all living cells — 1 mark
    • It supplies ATP — 1 mark

Quick check

  1. 1

    What type of reaction is respiration?

    1. AEndothermic
    2. BNeutral
    3. CExothermic
    4. DReversible
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    C: Exothermic

    Respiration transfers energy to the surroundings, so it is exothermic.

  2. 2

    Which of these is the word equation for aerobic respiration?

    1. AGlucose → lactic acid
    2. BGlucose + oxygen → carbon dioxide + water
    3. CCarbon dioxide + water → glucose + oxygen
    4. DGlucose → ethanol + carbon dioxide
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    B: Glucose + oxygen → carbon dioxide + water

    Glucose reacts with oxygen to make carbon dioxide and water.

  3. 3

    What is made when muscles respire anaerobically?

    1. AEthanol
    2. BCarbon dioxide and water
    3. COxygen
    4. DLactic acid
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    D: Lactic acid

    Anaerobic respiration in muscles makes lactic acid.

  4. 4

    What is made when yeast respires anaerobically?

    1. AEthanol and carbon dioxide
    2. BLactic acid
    3. CCarbon dioxide and water
    4. DGlucose and oxygen
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    A: Ethanol and carbon dioxide

    Yeast makes ethanol and carbon dioxide. This is called fermentation.

  5. 5

    Why does anaerobic respiration transfer less energy than aerobic respiration?

    1. AIt happens in the nucleus
    2. BGlucose is only partly oxidised
    3. CIt uses more oxygen
    4. DIt makes more water
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    B: Glucose is only partly oxidised

    The oxidation of glucose is incomplete, so less energy is transferred.

  6. 6

    Where do most of the reactions of aerobic respiration take place?

    1. AIn the nucleus
    2. BIn the ribosomes
    3. CIn the mitochondria
    4. DIn the cell wall
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    C: In the mitochondria

    Aerobic respiration takes place mainly in the mitochondria.

  7. 7

    Which of these is NOT a use of the energy transferred by respiration?

    1. AProducing light
    2. BMovement
    3. CBuilding larger molecules
    4. DKeeping warm
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    A: Producing light

    Organisms use the energy to build larger molecules, to move and to keep warm. Respiration does not make light.

  8. 8

    Which statement about respiration is correct?

    1. AIt only happens in animals
    2. BIt only happens in the day
    3. CIt only happens during exercise
    4. DIt happens in all living cells all the time
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    D: It happens in all living cells all the time

    Respiration is continuously occurring in all living cells, both plant and animal.

  9. 9

    What does respiration supply in all living cells?

    1. AOxygen
    2. BATP
    3. CChlorophyll
    4. DStarch
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    B: ATP

    Respiration transfers the energy from glucose to make ATP, which carries energy to where it is needed.

  10. 10

    Roughly how much ATP does aerobic respiration make per glucose compared with anaerobic respiration?

    1. AThe same amount
    2. BA little less
    3. CHalf as much
    4. DMany times more
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    D: Many times more

    Aerobic respiration makes about 30 ATP per glucose and anaerobic respiration only 2, so the yield is many times greater.

Monomers and Polymers

Just this lesson
  1. 1 State [2 marks]

    State what is meant by the terms monomer and polymer.

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    Model answer

    A monomer is a small molecule that can join with others. A polymer is a large molecule made of many monomers joined together.

    Mark scheme

    • Monomer: small molecule that joins with others — 1 mark
    • Polymer: large molecule made of many monomers — 1 mark
  2. 2 Name [3 marks]

    Figure 1 shows how large molecules are made from small ones. Name the molecules shown at A, B and C.

    Three rows of molecules joining, with three parts marked A, B and C.
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    Model answer

    A is sugars (glucose). B is a protein. C is a lipid.

    Mark scheme

    • A: sugars / glucose — 1 mark
    • B: protein — 1 mark
    • C: lipid — 1 mark
  3. 3 State [3 marks]

    Figure 2 shows how a lipid molecule is made. Name the parts shown at A, B and C.

    A lipid being made from glycerol and fatty acids, with three parts marked A, B and C.
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    Model answer

    A is glycerol. B is a fatty acid. C is a lipid (molecule).

    Mark scheme

    • A: glycerol — 1 mark
    • B: fatty acid — 1 mark
    • C: lipid — 1 mark
  4. 4 Describe [3 marks]

    Describe how proteins are made in a cell and how they are broken down.

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    Model answer

    Amino acids are joined together in a chain to make a protein. This needs energy from respiration and is controlled by enzymes. Protease enzymes break proteins down into amino acids.

    Mark scheme

    • Amino acids are joined to make proteins — 1 mark
    • Uses energy / controlled by enzymes — 1 mark
    • Protease breaks proteins down into amino acids — 1 mark
  5. 5 Explain [3 marks]

    Explain why the starch in a slice of bread is broken down before the body can use it.

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    Model answer

    Starch is a polymer, a large molecule made of many sugars. It is too large to be absorbed. Amylase, a carbohydrase, breaks it down into sugars, which can be absorbed and used in respiration.

    Mark scheme

    • Starch is a large molecule / polymer — 1 mark
    • Amylase breaks it down into sugars — 1 mark
    • Sugars can be absorbed and used in respiration — 1 mark
  6. 6 Describe [4 marks]

    Describe how you would test a food sample for starch, sugars, protein and lipids.

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    Model answer

    Starch: add iodine solution, which turns blue-black. Sugars: heat with Benedict's reagent, which turns green, yellow, orange or brick-red. Protein: add biuret reagent, which turns purple. Lipids: shake with ethanol, then add water, and a cloudy white emulsion forms.

    Mark scheme

    • Starch with iodine, blue-black — 1 mark
    • Sugars with Benedict's reagent and heat — 1 mark
    • Protein with biuret, purple — 1 mark
    • Lipids with ethanol then water, cloudy emulsion — 1 mark
  7. 7 Explain [4 marks]

    Explain the importance of sugars, amino acids, fatty acids and glycerol in the body.

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    Model answer

    Sugars are joined to make carbohydrates such as glycogen and starch. Amino acids are joined to make proteins. Fatty acids and glycerol are joined to make lipids. They are also released when the large molecules are broken down, so they can be used again or in respiration.

    Mark scheme

    • Sugars make carbohydrates — 1 mark
    • Amino acids make proteins — 1 mark
    • Fatty acids and glycerol make lipids — 1 mark
    • They are released again when the molecules are broken down — 1 mark

Quick check

  1. 1

    What are the monomers that make a protein?

    1. ASugars
    2. BFatty acids
    3. CAmino acids
    4. DGlycerol
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    C: Amino acids

    Proteins are polymers made of amino acids.

  2. 2

    Which of these is a carbohydrate polymer used by plants to strengthen cell walls?

    1. AGlycogen
    2. BCellulose
    3. CLipase
    4. DGlycerol
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    B: Cellulose

    Cellulose is made from sugars and strengthens plant cell walls.

  3. 3

    What is a lipid made from?

    1. AThree glycerol and one fatty acid
    2. BAmino acids
    3. CSugars
    4. DOne glycerol and three fatty acids
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    D: One glycerol and three fatty acids

    A lipid is made from one molecule of glycerol and three molecules of fatty acids.

  4. 4

    Which enzyme breaks down proteins?

    1. AProtease
    2. BLipase
    3. CAmylase
    4. DCarbohydrase
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    A: Protease

    Protease breaks proteins down into amino acids.

  5. 5

    What do animals use to store glucose?

    1. AStarch
    2. BCellulose
    3. CGlycogen
    4. DLipid
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    C: Glycogen

    Animals store glucose as glycogen in the liver and muscles.

  6. 6

    What is the colour change in the biuret test when protein is present?

    1. ABlue to brick-red
    2. BBlue to purple
    3. COrange-brown to blue-black
    4. DClear to cloudy
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    B: Blue to purple

    Biuret reagent changes from blue to purple when protein is present.

  7. 7

    What does the synthesis of polymers need?

    1. ALight only
    2. BOxygen only
    3. CStarch only
    4. DEnzymes and energy from respiration
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    D: Enzymes and energy from respiration

    Joining monomers is controlled by enzymes and uses energy from respiration.

  8. 8

    Which pair of molecules is made when a lipid is broken down?

    1. AFatty acids and glycerol
    2. BAmino acids and sugars
    3. CSugars and glycerol
    4. DStarch and fatty acids
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    A: Fatty acids and glycerol

    Lipase breaks a lipid down into fatty acids and glycerol.

Food Tests

Just this lesson
  1. 1 Name [1 mark]

    Name the reagent used to test for starch.

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    Model answer

    Iodine solution.

    Mark scheme

    • Iodine (solution) — 1 mark
  2. 2 State [2 marks]

    State the colour change that shows that starch is present in a food sample.

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    Model answer

    Orange-brown to blue-black.

    Mark scheme

    • Starts orange-brown / yellow-brown — 1 mark
    • Changes to blue-black — 1 mark
  3. 3 Interpret [4 marks]

    Figure 1 shows four tests on a sample of food. Tube A contains iodine solution, tube B contains Benedict's reagent after heating, tube C contains Biuret reagent, and in tube D the food was shaken with ethanol and poured into water. Use Figure 1 to say whether the sample contains starch, sugar, protein and lipid.

    Four test tubes after food tests, with colours of orange-brown, brick red, purple and clear.
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    Model answer

    Starch is not present, because the iodine stayed orange-brown. Sugar is present, because Benedict's turned brick red. Protein is present, because Biuret turned purple. Lipid is not present, because the liquid stayed clear.

    Mark scheme

    • Starch absent: iodine stayed orange-brown — 1 mark
    • Sugar present: Benedict's brick red — 1 mark
    • Protein present: Biuret purple — 1 mark
    • Lipid absent: no cloudy emulsion — 1 mark
  4. 4 Describe [3 marks]

    Describe how you would test a food sample for sugar.

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    Model answer

    Add Benedict's reagent to the sample and heat it in a water bath. If sugar is present, the blue colour changes to green, yellow, orange or brick red.

    Mark scheme

    • Add Benedict's reagent — 1 mark
    • Heat in a (hot) water bath — 1 mark
    • Blue changes to green / yellow / orange / brick red if sugar is present — 1 mark
  5. 5 Suggest [2 marks]

    A student heats a test tube containing Benedict's reagent in a hot water bath, and not in a Bunsen burner flame. Suggest two reasons for this.

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    Model answer

    The liquid cannot boil over or spit out of the tube, so there is less risk of burns. The temperature is more even and easier to control.

    Mark scheme

    • Less risk of the liquid boiling over / spitting / burns — 1 mark
    • More even / controlled temperature — 1 mark
  6. 6 Describe [3 marks]

    Describe how you would test a food for lipids, and what you would see if the result is positive.

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    Model answer

    Shake the food with ethanol and pour the liquid into a test tube of water. If lipid is present, a cloudy white emulsion forms.

    Mark scheme

    • Shake the food with ethanol — 1 mark
    • Pour into water — 1 mark
    • A cloudy white emulsion forms if lipid is present — 1 mark
  7. 7 Explain [2 marks]

    A student also tests some distilled water with iodine solution. Explain why.

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    Model answer

    Distilled water contains no starch, so it acts as a control. It shows what a negative result looks like, so the student can tell that a colour change in the food sample is a real result.

    Mark scheme

    • It is a control / shows a negative result — 1 mark
    • To compare with the food sample / so a colour change can be recognised — 1 mark
  8. 8 Interpret [3 marks]

    Food X gave these results: the iodine solution turned blue-black, Benedict's reagent stayed blue after heating, Biuret reagent stayed blue, and the emulsion test stayed clear. Say what food X contains and does not contain.

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    Model answer

    Food X contains starch. It does not contain sugar, protein or lipid.

    Mark scheme

    • Contains starch — 1 mark
    • No sugar (Benedict's stayed blue) — 1 mark
    • No protein (Biuret stayed blue) and no lipid (stayed clear) — 1 mark
  9. 9 Describe [2 marks]

    Describe two safety precautions that should be taken when doing the food tests.

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    Model answer

    Wear eye protection (goggles). Use a hot water bath instead of a flame, and keep the ethanol away from flames.

    Mark scheme

    • Wear goggles / eye protection — 1 mark
    • Use a water bath / keep ethanol away from flames / hold hot tubes with a holder — 1 mark
  10. 10 Describe [6 marks]

    A student has three foods, P, Q and R. Describe how the student could use Benedict's reagent to find out which food contains the most sugar.

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    Model answer

    Prepare a solution of each food in the same way. Put the same volume of each sample in a test tube and add the same volume of Benedict's reagent. Heat all the tubes in the same hot water bath for the same length of time. Compare the colours. The food that goes closest to brick red contains the most sugar. Test distilled water as a control and repeat to check the results.

    Mark scheme

    • Prepare a solution of each food in the same way — 1 mark
    • Same volume of each sample and of Benedict's reagent — 1 mark
    • Heat in a water bath for the same time and temperature — 1 mark
    • Compare the colours — 1 mark
    • Closest to brick red = most sugar — 1 mark
    • Control with distilled water / repeat the test — 1 mark

Quick check

  1. 1

    Which reagent turns purple when protein is present?

    1. AIodine solution
    2. BBenedict's reagent
    3. CBiuret reagent
    4. DEthanol
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    C: Biuret reagent

    Biuret reagent changes from blue to purple when protein is present.

  2. 2

    A food is tested with iodine solution and the solution turns blue-black. What does the food contain?

    1. AStarch
    2. BSugar
    3. CProtein
    4. DLipid
    Show answerHide answer

    A: Starch

    Iodine turns blue-black when starch is present.

  3. 3

    What must be done after adding Benedict's reagent to a sample?

    1. AAdd iodine
    2. BLeave it in the freezer
    3. CAdd ethanol
    4. DHeat it in a water bath
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    D: Heat it in a water bath

    The mixture must be heated in a water bath. Without heating, the colour change will not happen.

  4. 4

    In Benedict's test, a sample turns brick red. What does this show?

    1. AThere is no sugar
    2. BThere is a large amount of sugar
    3. CThere is protein
    4. DThere is starch
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    B: There is a large amount of sugar

    Brick red is the last colour in the series from blue, and it shows a large amount of sugar.

  5. 5

    What is the positive result for the emulsion test for lipids?

    1. AA blue-black colour
    2. BA purple colour
    3. CA cloudy white emulsion
    4. DA brick-red colour
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    C: A cloudy white emulsion

    If lipid is present, the ethanol mixture forms a cloudy white emulsion when it is poured into water.

  6. 6

    Why is the lipid test done with ethanol?

    1. ALipids dissolve in ethanol
    2. BEthanol turns purple
    3. CEthanol is a protein
    4. DEthanol is a sugar
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    A: Lipids dissolve in ethanol

    Lipids dissolve in ethanol, but not in water, so the ethanol carries them into solution.

  7. 7

    Why should a student use a water bath instead of a flame to heat Benedict's reagent?

    1. AA flame makes the sugar disappear
    2. BBenedict's reagent only works in water
    3. CA flame turns it blue
    4. DThe liquid is less likely to boil over
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    D: The liquid is less likely to boil over

    The liquid is less likely to boil over, and the temperature is more even and safer.

  8. 8

    A food gives no colour change with any of the four tests. What can you conclude?

    1. AIt contains all four
    2. BIt contains none of the four groups tested for
    3. CIt contains only protein
    4. DIt contains only water
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    B: It contains none of the four groups tested for

    All four tests were negative, so the food contains none of starch, sugar, protein or lipid.

  9. 9

    Why is it useful to test a food that you know contains starch with iodine solution?

    1. AIt shows what a positive result looks like
    2. BIt makes the iodine work
    3. CIt removes the starch
    4. DIt cools the iodine
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    A: It shows what a positive result looks like

    It is a positive control, which shows what a positive result looks like for comparison.

  10. 10

    These tests are qualitative. What does this mean?

    1. AThey give an exact amount
    2. BThey only work at 75 °C
    3. CThey show whether a substance is present, not exactly how much
    4. DThey need a colorimeter
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    C: They show whether a substance is present, not exactly how much

    Qualitative tests show whether a substance is present, but they do not measure exactly how much there is.